Advanced PCR Designer

TCCTGAAAAGCAGACAGGTTTCTAACACTTTAGAAATAGAGTACATACATACTCCTGAGTGTATCATCTTTACTTTTTCCATGTTTATGCTGTGTTATAGCCATAACTGGGGAAATACATTTTTTCTGTAACATTATATTTGTATTTGTTTTTAGGGAGTGCAGCCTGCCACAGGCGAGGTTGTGTTTGATAGTTTCCAGGACTCTGCTTCTCGTTCAGAGCTAGAAACCCGGATGTCAAGCCTGCAGCCAGTAGAGCTGCTGCTTCCTTCGGCCTTGTCCGAGCAAACAGAGGCGCTCATCCACAGAGCCACATCTGTTAG Design Primers

An interactive web-based PCR primer design tool supporting multiple PCR types (standard, qPCR, ddPCR). Users input DNA sequences and adjust parameters (primer length, product size) via an intuitive interface. The tool generates primer pairs with real-time validation of thermodynamic properties (melting temperature, GC content) and specificity checks. Key features include:

Thermodynamic Calculations: Computes Tm using Wallace rule and GC percentage

PCR-Type Optimization: Auto-adjusts parameters for different PCR methodologies

Primer Validation: Checks Tm consistency and GC content ranges per PCR type

Specificity Detection: Identifies non-specific primers using sequence matching

Visual Feedback: Color-coded results highlight potential non-specific binding